Hotspot

trnC-GCA-petN

LSC · 27947-28878 · 932 bp

Back to Leucocoryne

Pi 0.00108

MarkerSeek score 60.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 1
pi: 0.00108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	1005-1013 bp	1.0	76.5
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	1057-1065 bp	1.0	75.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	1056-1064 bp	1.0	75.5
trnC-GCA-petN_p4	TTTTGGATCGAATTTGGCGG	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.4	58.0	1044-1052 bp	1.0	75.5
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	50.0	55.0	57.5	57.8	1058-1066 bp	1.0	75.1