Hotspot

rps16-trnQ-UUG

LSC · 5758-6820 · 1063 bp

Back to Leucocoryne

Pi 0.00284

MarkerSeek score 60.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1063
variable_sites: 3
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCGAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1153-1159 bp	1.0	79.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1130-1136 bp	1.0	78.9
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1141-1147 bp	1.0	78.4
rps16-trnQ-UUG_p4	CCACAGCGAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	1151-1157 bp	1.0	78.0
rps16-trnQ-UUG_p5	ATCCACAGCGAATCATGTCC	TTCGGAGGTTCGAATCCTTC	50.0	50.0	58.0	57.3	1157-1163 bp	1.0	75.0