Hotspot

ndhK

LSC · 48406-49083 · 678 bp

Back to Leucas

Pi 0.00885
MarkerSeek score 63.80
Primer available yes
Label ndhK

Feature Metadata

label_name
ndhK
feature_type
gene
region
LSC
length_bp
678
variable_sites
6
pi
0.00885
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK_p1 ACAAACGACCCTGCATCTTT GTTTATGCATGGCGAAAGGG 45.0 50.0 58.0 57.8 879 bp 1.0 78.7
ndhK_p2 TCAAGAAGATTGACCCGCTG GTTTATGCATGGCGAAAGGG 50.0 50.0 57.9 57.8 817 bp 1.0 78.1
ndhK_p3 ACAAACGACCCTGCATCTTT GGGGCATTGGAATGGTCTTA 45.0 50.0 58.0 57.5 861 bp 1.0 76.8
ndhK_p4 CGCTGCTTGTTGTTCTGTAC GTTTATGCATGGCGAAAGGG 50.0 50.0 57.7 57.8 802 bp 1.0 76.5
ndhK_p5 TCAAGAAGATTGACCCGCTG GGGGCATTGGAATGGTCTTA 50.0 50.0 57.9 57.5 799 bp 1.0 76.2