Hotspot

ndhF

SSC · 108016-110238 · 2223 bp

Pi 0.013

MarkerSeek score 50.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 29
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	ATGCGTGAACAGTATGGCAA	50.0	45.0	58.0	58.2	2821 bp	1.0	79.3
ndhF_p2	AAGAGGGATCCACCGAAGAA	ATGCGTGAACAGTATGGCAA	50.0	45.0	58.0	58.2	2813 bp	1.0	79.3
ndhF_p3	GGGAGAAAGGGTGGAAAGTG	ATGCGTGAACAGTATGGCAA	55.0	45.0	58.1	58.2	2893 bp	1.0	79.0
ndhF_p4	AGGGTGGAAAGTGAGGAAGA	ATGCGTGAACAGTATGGCAA	50.0	45.0	57.9	58.2	2886 bp	1.0	78.7
ndhF_p5	ACAGCTTCCGAAATGAAGGG	ATGCGTGAACAGTATGGCAA	50.0	45.0	58.2	58.2	2847 bp	1.0	78.3