Hotspot

atpF

LSC · 11875-13092 · 1218 bp

Back to Leucas

Pi 0.00996
MarkerSeek score 65.50
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1218
variable_sites
12
pi
0.00996
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 TGCCTTAGGGTAGATAATACT AAAAAGAGGGGCAAAGTGGT 38.1 45.0 52.5 57.8 1343-1350 bp 1.0 41.5
atpF_p2 TGCCTTAGGGTAGATAATACT AAGAGGGGCAAAGTGGTAGA 38.1 50.0 52.5 58.3 1340-1347 bp 1.0 41.5
atpF_p3 TGCCTTAGGGTAGATAATACT AGAGGGGCAAAGTGGTAGAA 38.1 50.0 52.5 58.3 1339-1346 bp 1.0 41.5
atpF_p4 ATGCCTTAGGGTAGATAATACT AAAAAGAGGGGCAAAGTGGT 36.4 45.0 53.2 57.8 1344-1351 bp 1.0 41.5
atpF_p5 ATGCCTTAGGGTAGATAATACT AGAGGGGCAAAGTGGTAGAA 36.4 50.0 53.2 58.3 1340-1347 bp 1.0 41.5