Hotspot

ndhH-rps15

SSC · 120437-120536 · 100 bp

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Pi 0.04
MarkerSeek score 74.10
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
100
variable_sites
4
pi
0.04
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 AACACCATGCATTGATGGGT GAAAACGTCAACGACTGCTG 45.0 50.0 58.1 58.0 276 bp 1.0 80.6
ndhH-rps15_p2 AACACCATGCATTGATGGGT TTGGGAAAACGTCAACGACT 45.0 45.0 58.1 58.0 280 bp 1.0 80.5
ndhH-rps15_p3 CACCGTCGAGAGTAACGATC GAAAACGTCAACGACTGCTG 55.0 50.0 57.9 58.0 304 bp 1.0 80.3
ndhH-rps15_p4 CACCGTCGAGAGTAACGATC TTGGGAAAACGTCAACGACT 55.0 45.0 57.9 58.0 308 bp 1.0 80.1
ndhH-rps15_p5 CTTCACCGTCGAGAGTAACG GAAAACGTCAACGACTGCTG 55.0 50.0 58.1 58.0 307 bp 1.0 80.0