Hotspot

trnL-UAG-ccsA

SSC · 111720-111804 · 85 bp

Back to Leucas

Pi 0.0353
MarkerSeek score 61.60
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
85
variable_sites
3
pi
0.0353
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TGCTCTAAGGTTGAAAATATCA 50.0 31.8 57.9 53.2 169 bp 1.0 41.4
trnL-UAG-ccsA_p2 GGTAGACACGCTGCTCTTAG TGCTCTAAGGTTGAAAATATCA 55.0 31.8 57.8 53.2 171 bp 1.0 41.4
trnL-UAG-ccsA_p3 GTAGACACGCTGCTCTTAGG TGCTCTAAGGTTGAAAATATCA 55.0 31.8 57.8 53.2 170 bp 1.0 41.4
trnL-UAG-ccsA_p4 GTGAAATCGGTAGACACGCT TGCTCTAAGGTTGAAAATATCA 50.0 31.8 58.0 53.2 179 bp 1.0 41.3
trnL-UAG-ccsA_p5 TGAAATCGGTAGACACGCTG TGCTCTAAGGTTGAAAATATCA 50.0 31.8 58.0 53.2 178 bp 1.0 41.3