Hotspot

rpl32-trnL-UAG

SSC · 110925-111639 · 715 bp

Back to Leucas

Pi 0.0241
MarkerSeek score 66.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
715
variable_sites
17
pi
0.0241
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCTACCGGGAATTCA AGCGTGTCTACCGATTTCAC 42.9 50.0 56.1 58.0 813-824 bp 1.0 59.2
rpl32-trnL-UAG_p2 TCTCTTTCTACCGGGAATTCA CAGCGTGTCTACCGATTTCA 42.9 50.0 56.1 58.0 814-825 bp 1.0 59.2
rpl32-trnL-UAG_p3 GGTAAATCTCTTTCTACCGGGA AGCGTGTCTACCGATTTCAC 45.5 50.0 57.0 58.0 819-830 bp 1.0 58.3
rpl32-trnL-UAG_p4 GGTAAATCTCTTTCTACCGGGA CAGCGTGTCTACCGATTTCA 45.5 50.0 57.0 58.0 820-831 bp 1.0 58.3
rpl32-trnL-UAG_p5 AGGTAAATCTCTTTCTACCGGG AGCGTGTCTACCGATTTCAC 45.5 50.0 57.0 58.0 820-831 bp 1.0 58.3