Hotspot

petA-psbJ

LSC · 62025-63014 · 990 bp

Back to Leucas

Pi 0.0173
MarkerSeek score 60.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
990
variable_sites
17
pi
0.0173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1047-1056 bp 1.0 76.7
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1049-1058 bp 1.0 76.7
petA-psbJ_p3 GAGAAGGTTCAATTGGCCGA CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1046-1055 bp 1.0 76.2
petA-psbJ_p4 TCGAGAAGGTTCAATTGGCC CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1048-1057 bp 1.0 76.2
petA-psbJ_p5 AGAAGGTTCAATTGGCCGAA TCCGGATTAGGTTCATCCCT 45.0 50.0 57.7 57.5 1046-1055 bp 1.0 75.8