Hotspot

trnF-GAA-ndhJ

LSC · 47172-47834 · 663 bp

Back to Leucas

Pi 0.0201
MarkerSeek score 63.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
663
variable_sites
13
pi
0.0201
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 790-805 bp 1.0 82.1
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 754-769 bp 1.0 81.8
trnF-GAA-ndhJ_p3 CTGGTAGAGCAGAGGACTGA AGTTGGATAGGATGGCCCTT 55.0 50.0 57.9 58.1 778-793 bp 1.0 81.1
trnF-GAA-ndhJ_p4 GGGATAGCTCAGCTGGTAGA GATAATCATCCGCGCCTGAA 55.0 50.0 58.0 58.2 829-844 bp 1.0 81.0
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA GATAATCATCCGCGCCTGAA 50.0 50.0 58.1 58.2 793-808 bp 1.0 80.7