Hotspot

trnM-CAU-rps14

LSC · 36153-36301 · 149 bp

Back to Leucas

Pi 0.0403
MarkerSeek score 69.20
Primer available yes
Label trnM-CAU-rps14

Feature Metadata

label_name
trnM-CAU-rps14
feature_type
igs
region
LSC
length_bp
149
variable_sites
6
pi
0.0403
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-rps14_p1 GCTACCAAACTGCTCTACCC CGTTGTTTTTCGACCGGAAG 55.0 50.0 58.0 58.0 293 bp 1.0 81.3
trnM-CAU-rps14_p2 AAGGTTATGAGCCTTGCGAG CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 312 bp 1.0 81.2
trnM-CAU-rps14_p3 GCTACCAAACTGCTCTACCC CGCGAGCTAACTATCGAGAC 55.0 55.0 58.0 58.1 271 bp 1.0 81.1
trnM-CAU-rps14_p4 GATGTTGCGGAGACAGGATT CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 347 bp 1.0 81.0
trnM-CAU-rps14_p5 TTTGAACCCGTGACCTCAAG CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 329 bp 1.0 81.0