Hotspot

trnS-GCU-trnS-CGA

LSC · 8468-9154 · 687 bp

Back to Leucas

Pi 0.0196
MarkerSeek score 67.60
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
687
variable_sites
13
pi
0.0196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 727-742 bp 1.0 65.3
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 726-741 bp 1.0 65.3
trnS-GCU-trnS-CGA_p3 ACGGAAAGAGAGGGATTCGA ACCACTAAACTATACCCGCT 50.0 45.0 58.2 55.6 781-796 bp 1.0 63.8
trnS-GCU-trnS-CGA_p4 AGCAATCCGACGCTTTAGTC ACCACTAAACTATACCCGCT 50.0 45.0 58.4 55.6 729-744 bp 1.0 62.4
trnS-GCU-trnS-CGA_p5 CGACGCTTTAGTCCACTCAG ACCACTAAACTATACCCGCT 55.0 45.0 58.4 55.6 722-737 bp 1.0 62.3