Hotspot

rps16-trnQ-UUG

LSC · 6215-7166 · 952 bp

Back to Leucas

Pi 0.0133
MarkerSeek score 60.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
952
variable_sites
12
pi
0.0133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 990-1042 bp 1.0 83.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 973-1025 bp 1.0 83.2
rps16-trnQ-UUG_p3 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 991-1043 bp 1.0 82.7
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 988-1040 bp 1.0 81.4
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 987-1039 bp 1.0 81.4