Hotspot

ycf1

SSC · 121090-126192 · 5103 bp

Back to Leucanthemum

Pi 0.218

MarkerSeek score 74.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5103
variable_sites: 1666
pi: 0.218
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCAAGCGGCTCCCATTATT	TTGGACGGGGATGTCTTTTG	50.0	50.0	58.0	58.1	1044 bp	0.5	59.2
ycf1_p2	CTCAAGCGGCTCCCATTATT	TTTGATTGGACGGGGATGTC	50.0	50.0	58.0	57.9	1049 bp	0.5	59.0
ycf1_p3	TCGCAATTCTCTACGACGTC	TTGGACGGGGATGTCTTTTG	50.0	50.0	57.8	58.1	805 bp	0.5	58.2
ycf1_p4	CGCAATTCTCTACGACGTCT	TTGGACGGGGATGTCTTTTG	50.0	50.0	57.8	58.1	804 bp	0.5	58.1
ycf1_p5	TCGCAATTCTCTACGACGTC	TTTGATTGGACGGGGATGTC	50.0	50.0	57.8	57.9	810 bp	0.5	58.0