Hotspot

rpl22

LSC · 81864-82331 · 468 bp

Back to Leucanthemum

Pi 0.0823

MarkerSeek score 81.60

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 468
variable_sites: 77
pi: 0.0823
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TCTTCTTGCAGACCCTCAGA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1221-1224 bp	0.75	70.3
rpl22_p2	TCTTCTTGCAGACCCTCAGA	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	828-830 bp	0.75	70.1
rpl22_p3	TCTTCTTGCAGACCCTCAGA	AACCCTGTAGACCATCCACA	50.0	50.0	58.0	58.0	1159-1162 bp	0.75	70.0
rpl22_p4	TTTGTTCCATCGCCGGATAG	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.1	58.0	1820-1823 bp	0.75	69.9
rpl22_p5	TCTTCTTGCAGACCCTCAGA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1122-1125 bp	0.75	69.9