Hotspot

rpl16

LSC · 79570-81045 · 1476 bp

Back to Leucanthemum

Pi 0.09

MarkerSeek score 84.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 264
pi: 0.09
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	AATTGAAAGTCGACCCCGAG	50.0	50.0	58.0	57.9	2448-2477 bp	0.75	70.3
rpl16_p2	ACACATCAATTCTCGAGCCC	AATTGAAAGTCGACCCCGAG	50.0	50.0	58.0	57.9	2208-2237 bp	0.75	70.3
rpl16_p3	TCGACGATTGCTAGCTCCTA	AATTGAAAGTCGACCCCGAG	50.0	50.0	58.0	57.9	2238-2267 bp	0.75	70.2
rpl16_p4	GCGTCTTTGATCACAGCAAC	AATTGAAAGTCGACCCCGAG	50.0	50.0	58.0	57.9	2282-2311 bp	0.75	70.1
rpl16_p5	ACTCGAACCACGCTTTTTCT	AATTGAAAGTCGACCCCGAG	45.0	50.0	58.1	57.9	2775-2804 bp	0.75	70.0