Hotspot

ndhE

SSC · 115031-115336 · 306 bp

Back to Leucanthemum

Pi 0.32

MarkerSeek score 66.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 147
pi: 0.32
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ACAGCCAACACAATCCTCTG	TGGAGTAACTTCCGTGGTCT	50.0	50.0	58.1	58.0	1123-1134 bp	0.5	59.5
ndhE_p2	CAGCCAACACAATCCTCTGT	TGGAGTAACTTCCGTGGTCT	50.0	50.0	58.1	58.0	1122-1133 bp	0.5	59.5
ndhE_p3	GGCTCAAGGAGAGGTTTCTG	TGGAGTAACTTCCGTGGTCT	55.0	50.0	57.9	58.0	1243-1254 bp	0.5	59.4
ndhE_p4	AAGGAGAGGTTTCTGCAACG	TGGAGTAACTTCCGTGGTCT	50.0	50.0	58.1	58.0	1238-1249 bp	0.5	59.3
ndhE_p5	ACATCCGTCCCAAGGTATCA	TGGAGTAACTTCCGTGGTCT	50.0	50.0	58.1	58.0	1072-1083 bp	0.5	59.2