Hotspot

clpP1

LSC · 68418-70441 · 2024 bp

Back to Leucanthemum

Pi 0.0783

MarkerSeek score 83.10

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2024
variable_sites: 315
pi: 0.0783
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	AAACTCGGCAGGGTGAATTT	CAATGGGGAGGTTAATGCGA	45.0	50.0	58.0	57.9	2312-2328 bp	0.75	70.2
clpP1_p2	AACTCGGCAGGGTGAATTTT	CAATGGGGAGGTTAATGCGA	45.0	50.0	58.0	57.9	2311-2327 bp	0.75	70.2
clpP1_p3	AAACTCGGCAGGGTGAATTT	ACGAACATACCCTGTCTCCA	45.0	50.0	58.0	58.1	2710-2726 bp	0.75	70.0
clpP1_p4	AACTCGGCAGGGTGAATTTT	ACGAACATACCCTGTCTCCA	45.0	50.0	58.0	58.1	2709-2725 bp	0.75	70.0
clpP1_p5	CACATTCTTCGGCTCTACCC	CAATGGGGAGGTTAATGCGA	55.0	50.0	58.1	57.9	2908-2924 bp	0.75	69.7