Hotspot

ndhF-rpl32

SSC · 109436-110468 · 1033 bp

Back to Leucanthemum

Pi 0.234

MarkerSeek score 79.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1033
variable_sites: 358
pi: 0.234
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGGGAAATTGTGCGGATTT	CCCCACCACCAAAATCTGAA	45.0	50.0	58.1	58.0	2264 bp	0.5	61.2
ndhF-rpl32_p2	GGGAAATTGTGCGGATTTCG	CCCCACCACCAAAATCTGAA	50.0	50.0	58.1	58.0	2262 bp	0.5	61.0
ndhF-rpl32_p3	AACAGGTCGTGTGAACCAAA	CCCCACCACCAAAATCTGAA	45.0	50.0	57.9	58.0	2037 bp	0.5	61.0
ndhF-rpl32_p4	ACAGGTCGTGTGAACCAAAA	CCCCACCACCAAAATCTGAA	45.0	50.0	57.9	58.0	2036 bp	0.5	61.0
ndhF-rpl32_p5	AAGGGGAAATTGTGCGGATT	CCCCACCACCAAAATCTGAA	45.0	50.0	58.1	58.0	2265 bp	0.25	49.4