Hotspot

psbB-psbT

LSC · 72418-72604 · 187 bp

Back to Leucanthemum

Pi 0.0998

MarkerSeek score 80.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 37
pi: 0.0998
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGAGCATTCCAAAAACTTGG	GGAATGTATAAACCAATGCTTCCA	45.0	37.5	55.4	57.5	258-263 bp	1.0	52.0
psbB-psbT_p2	GGAGCATTCCAAAAACTTGG	AGGAATGTATAAACCAATGCTTCC	45.0	37.5	55.4	57.3	259-264 bp	1.0	52.0
psbB-psbT_p3	GGAGCATTCCAAAAACTTGG	GGAATGTATAAACCAATGCTTCCAT	45.0	36.0	55.4	57.9	258-263 bp	1.0	52.0
psbB-psbT_p4	GGAGCATTCCAAAAACTTGG	GGAATGTATAAACCAATGCTTCC	45.0	39.1	55.4	55.9	258-263 bp	1.0	52.0
psbB-psbT_p5	GGAGCATTCCAAAAACTTGG	GAGGAATGTATAAACCAATGCTTCC	45.0	40.0	55.4	58.3	260-265 bp	1.0	51.9