Hotspot

psaJ-rpl33

LSC · 65665-66081 · 417 bp

Back to Leucanthemum

Pi 0.144

MarkerSeek score 80.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 119
pi: 0.144
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGAACTGAACTAAGAGCGCT	TCGTTGTTGCTTCAAGGTCA	50.0	45.0	58.0	58.0	1690 bp	0.75	69.9
psaJ-rpl33_p2	GTTTTGGAGACCCACGTTCT	TCGTTGTTGCTTCAAGGTCA	50.0	45.0	58.0	58.0	1712 bp	0.75	69.7
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	TCGTTGTTGCTTCAAGGTCA	50.0	45.0	58.0	58.0	1093 bp	0.75	69.7
psaJ-rpl33_p4	CGAACTGAACTAAGAGCGCT	GTAAGATAAGGGGCAGGTGC	50.0	55.0	58.0	58.0	1365 bp	0.5	59.7
psaJ-rpl33_p5	GTTTTGGAGACCCACGTTCT	GTAAGATAAGGGGCAGGTGC	50.0	55.0	58.0	58.0	1387 bp	0.5	59.6