Hotspot

rbcL-accD

LSC · 56039-56500 · 462 bp

Back to Leucanthemum

Pi 0.0973

MarkerSeek score 81.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 89
pi: 0.0973
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTGTACTACAGTTCGGTGGC	CAATAGGACCAAGACTGCCC	55.0	55.0	58.0	58.0	873-874 bp	0.75	70.0
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	CAATAGGACCAAGACTGCCC	50.0	55.0	58.0	58.0	1348-1349 bp	0.75	69.9
rbcL-accD_p3	CTAACAGGGGGATTCACTGC	CAATAGGACCAAGACTGCCC	55.0	55.0	58.0	58.0	1258-1259 bp	0.75	69.7
rbcL-accD_p4	AATGCTACTGCGGGTACATG	CAATAGGACCAAGACTGCCC	50.0	55.0	58.0	58.0	1345-1346 bp	0.75	69.7
rbcL-accD_p5	GCGGGTACATGTGAAGACAT	CAATAGGACCAAGACTGCCC	50.0	55.0	58.0	58.0	1336 bp	0.5	59.6