primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	rbcL-accD	1	CTGTACTACAGTTCGGTGGC	CAATAGGACCAAGACTGCCC	20	20	55.0	55.0	58.0	58.0	7.9	0.0	0.051953	56039	56500	873-874	0.750000	70.0
rbcL-accD_p2	rbcL-accD	2	TTGAATGCTACTGCGGGTAC	CAATAGGACCAAGACTGCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.062665	56039	56500	1348-1349	0.750000	69.9
rbcL-accD_p3	rbcL-accD	3	CTAACAGGGGGATTCACTGC	CAATAGGACCAAGACTGCCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.088245	56039	56500	1258-1259	0.750000	69.7
rbcL-accD_p4	rbcL-accD	4	AATGCTACTGCGGGTACATG	CAATAGGACCAAGACTGCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.090564	56039	56500	1345-1346	0.750000	69.7
rbcL-accD_p5	rbcL-accD	5	GCGGGTACATGTGAAGACAT	CAATAGGACCAAGACTGCCC	20	20	50.0	55.0	58.0	58.0	9.1	0.0	0.068298	56039	56500	1336-1336	0.500000	59.6
ycf4_p1	ycf4	1	GCAATCGCAATGGCTTCTTT	GGAATTCCTGAGCCCAAGAG	20	20	45.0	55.0	58.0	58.0	0.0	17.7	0.050318	58964	59518	1643-1644	0.750000	69.9
ycf4_p2	ycf4	2	GCAATCGCAATGGCTTCTTT	TGAGAGCGAAATCCACCAAG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.099570	58964	59518	1297-1298	0.750000	69.6
ycf4_p3	ycf4	3	GCAATCGCAATGGCTTCTTT	TGCTTTGACTCGAAGGGATG	20	20	45.0	50.0	58.0	57.9	0.0	8.0	0.099570	58964	59518	1084-1085	0.750000	69.6
ycf4_p4	ycf4	4	GCAATCGCAATGGCTTCTTT	GCTTTGACTCGAAGGGATGT	20	20	45.0	50.0	58.0	57.9	0.0	8.0	0.101034	58964	59518	1083-1084	0.750000	69.6
ycf4_p5	ycf4	5	ATAGACCCAAATTGGCGGAC	GGAATTCCTGAGCCCAAGAG	20	20	50.0	55.0	57.9	58.0	6.5	17.7	0.102671	58964	59518	1473-1474	0.750000	69.5
petA-psbJ_p1	petA-psbJ	1	GTAGGCGGAAACAGAGGAAG	TCCCTATTCATTGCGGGTTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.062874	61668	62367	1818-2245	1.000000	88.7
petA-psbJ_p2	petA-psbJ	2	CTGACGGGAGCAAGAGTAAC	TTAGGAGGCCTCAATGACCA	20	20	55.0	50.0	58.0	58.0	0.0	33.3	0.036917	61668	62367	1620-1686	0.750000	70.2
petA-psbJ_p3	petA-psbJ	3	GTAGGCGGAAACAGAGGAAG	TTAGGAGGCCTCAATGACCA	20	20	55.0	50.0	58.0	58.0	0.0	33.3	0.046126	61668	62367	1654-1720	0.750000	70.2
petA-psbJ_p4	petA-psbJ	4	CTGACGGGAGCAAGAGTAAC	TCCCTATTCATTGCGGGTTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.053665	61668	62367	1784-1850	0.750000	70.1
petA-psbJ_p5	petA-psbJ	5	CTGACGGGAGCAAGAGTAAC	TGGAGGGAAGTGGGGTAAAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.071162	61668	62367	1218-1284	0.750000	70.1
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	64967	65141	257-263	1.000000	89.1
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	64967	65141	258-264	1.000000	89.1
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	64967	65141	246-252	1.000000	89.1
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	64967	65141	244-250	1.000000	89.1
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	64967	65141	247-253	1.000000	89.1
psaJ-rpl33_p1	psaJ-rpl33	1	CGAACTGAACTAAGAGCGCT	TCGTTGTTGCTTCAAGGTCA	20	20	50.0	45.0	58.0	58.0	11.3	11.5	0.047286	65665	66081	1690-1690	0.750000	69.9
psaJ-rpl33_p2	psaJ-rpl33	2	GTTTTGGAGACCCACGTTCT	TCGTTGTTGCTTCAAGGTCA	20	20	50.0	45.0	58.0	58.0	0.0	11.5	0.068086	65665	66081	1712-1712	0.750000	69.7
psaJ-rpl33_p3	psaJ-rpl33	3	ACATATCTTTCCGTGGCACC	TCGTTGTTGCTTCAAGGTCA	20	20	50.0	45.0	58.0	58.0	12.9	11.5	0.069645	65665	66081	1093-1093	0.750000	69.7
psaJ-rpl33_p4	psaJ-rpl33	4	CGAACTGAACTAAGAGCGCT	GTAAGATAAGGGGCAGGTGC	20	20	50.0	55.0	58.0	58.0	11.3	0.0	0.047323	65665	66081	1365-1365	0.500000	59.7
psaJ-rpl33_p5	psaJ-rpl33	5	GTTTTGGAGACCCACGTTCT	GTAAGATAAGGGGCAGGTGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.068124	65665	66081	1387-1387	0.500000	59.6
clpP1_p1	clpP1	1	AAACTCGGCAGGGTGAATTT	CAATGGGGAGGTTAATGCGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.055958	68418	70441	2312-2328	0.750000	70.2
clpP1_p2	clpP1	2	AACTCGGCAGGGTGAATTTT	CAATGGGGAGGTTAATGCGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.055958	68418	70441	2311-2327	0.750000	70.2
clpP1_p3	clpP1	3	AAACTCGGCAGGGTGAATTT	ACGAACATACCCTGTCTCCA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.071602	68418	70441	2710-2726	0.750000	70.0
clpP1_p4	clpP1	4	AACTCGGCAGGGTGAATTTT	ACGAACATACCCTGTCTCCA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.071602	68418	70441	2709-2725	0.750000	70.0
clpP1_p5	clpP1	5	CACATTCTTCGGCTCTACCC	CAATGGGGAGGTTAATGCGA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.110009	68418	70441	2908-2924	0.750000	69.7
psbB-psbT_p1	psbB-psbT	1	GGAGCATTCCAAAAACTTGG	GGAATGTATAAACCAATGCTTCCA	20	24	45.0	37.5	55.4	57.5	0.0	0.0	7.046773	72418	72604	258-263	1.000000	52.0
psbB-psbT_p2	psbB-psbT	2	GGAGCATTCCAAAAACTTGG	AGGAATGTATAAACCAATGCTTCC	20	24	45.0	37.5	55.4	57.3	0.0	0.0	7.298125	72418	72604	259-264	1.000000	52.0
psbB-psbT_p3	psbB-psbT	3	GGAGCATTCCAAAAACTTGG	GGAATGTATAAACCAATGCTTCCAT	20	25	45.0	36.0	55.4	57.9	0.0	0.0	7.637269	72418	72604	258-263	1.000000	52.0
psbB-psbT_p4	psbB-psbT	4	GGAGCATTCCAAAAACTTGG	GGAATGTATAAACCAATGCTTCC	20	23	45.0	39.1	55.4	55.9	0.0	0.0	7.649158	72418	72604	258-263	1.000000	52.0
psbB-psbT_p5	psbB-psbT	5	GGAGCATTCCAAAAACTTGG	GAGGAATGTATAAACCAATGCTTCC	20	25	45.0	40.0	55.4	58.3	0.0	0.0	7.843667	72418	72604	260-265	1.000000	51.9
rpl16_p1	rpl16	1	CGCACCAAAAACTCGAGTTC	AATTGAAAGTCGACCCCGAG	20	20	50.0	50.0	58.0	57.9	29.8	13.0	0.113479	79570	81045	2448-2477	0.750000	70.3
rpl16_p2	rpl16	2	ACACATCAATTCTCGAGCCC	AATTGAAAGTCGACCCCGAG	20	20	50.0	50.0	58.0	57.9	7.8	13.0	0.124600	79570	81045	2208-2237	0.750000	70.3
rpl16_p3	rpl16	3	TCGACGATTGCTAGCTCCTA	AATTGAAAGTCGACCCCGAG	20	20	50.0	50.0	58.0	57.9	17.2	13.0	0.131590	79570	81045	2238-2267	0.750000	70.2
rpl16_p4	rpl16	4	GCGTCTTTGATCACAGCAAC	AATTGAAAGTCGACCCCGAG	20	20	50.0	50.0	58.0	57.9	3.9	13.0	0.139000	79570	81045	2282-2311	0.750000	70.1
rpl16_p5	rpl16	5	ACTCGAACCACGCTTTTTCT	AATTGAAAGTCGACCCCGAG	20	20	45.0	50.0	58.1	57.9	0.0	13.0	0.145297	79570	81045	2775-2804	0.750000	70.0
rpl22_p1	rpl22	1	TCTTCTTGCAGACCCTCAGA	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.018658	81864	82331	1221-1224	0.750000	70.3
rpl22_p2	rpl22	2	TCTTCTTGCAGACCCTCAGA	TCCCGGGCATCTACCATTAT	20	20	50.0	50.0	58.0	58.0	0.0	18.5	0.054319	81864	82331	828-830	0.750000	70.1
rpl22_p3	rpl22	3	TCTTCTTGCAGACCCTCAGA	AACCCTGTAGACCATCCACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054319	81864	82331	1159-1162	0.750000	70.0
rpl22_p4	rpl22	4	TTTGTTCCATCGCCGGATAG	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.1	58.0	5.0	29.9	0.055097	81864	82331	1820-1823	0.750000	69.9
rpl22_p5	rpl22	5	TCTTCTTGCAGACCCTCAGA	GGGCCCCAATTGGTAGAAAA	20	20	50.0	50.0	58.0	58.1	0.0	22.9	0.069876	81864	82331	1122-1125	0.750000	69.9
ndhF-rpl32_p1	ndhF-rpl32	1	AGGGGAAATTGTGCGGATTT	CCCCACCACCAAAATCTGAA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.071051	109436	110468	2264-2264	0.500000	61.2
ndhF-rpl32_p2	ndhF-rpl32	2	GGGAAATTGTGCGGATTTCG	CCCCACCACCAAAATCTGAA	20	20	50.0	50.0	58.1	58.0	11.0	0.0	0.100177	109436	110468	2262-2262	0.500000	61.0
ndhF-rpl32_p3	ndhF-rpl32	3	AACAGGTCGTGTGAACCAAA	CCCCACCACCAAAATCTGAA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.125069	109436	110468	2037-2037	0.500000	61.0
ndhF-rpl32_p4	ndhF-rpl32	4	ACAGGTCGTGTGAACCAAAA	CCCCACCACCAAAATCTGAA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.125069	109436	110468	2036-2036	0.500000	61.0
ndhF-rpl32_p5	ndhF-rpl32	5	AAGGGGAAATTGTGCGGATT	CCCCACCACCAAAATCTGAA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.071051	109436	110468	2265-2265	0.250000	49.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCGGCATTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.086679	110634	111587	1071-1104	0.500000	61.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCGGCATTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.088049	110634	111587	1072-1105	0.500000	61.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAGGATATTGGGCGGCATT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	12.5	0.0	0.094441	110634	111587	1082-1115	0.500000	61.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAGGATATTGGGCGGCATT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	12.5	0.0	0.095811	110634	111587	1083-1116	0.500000	61.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCGGCATTAAAAGCTTTGT	CCCGAATCAATCCAACGAGT	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.103024	110634	111587	1419-1452	0.500000	61.0
ndhD_p1	ndhD	1	GTAGTGATTCACGCGGTTCT	CATGTGTGCCACGTAAGGTA	20	20	50.0	50.0	58.0	57.9	12.5	0.0	0.095331	112945	114447	2169-2169	0.500000	59.3
ndhD_p2	ndhD	2	GTAGTGATTCACGCGGTTCT	TGTGTGCCACGTAAGGTATG	20	20	50.0	50.0	58.0	57.9	12.5	0.0	0.095331	112945	114447	2167-2167	0.500000	59.3
ndhD_p3	ndhD	3	GTAGTGATTCACGCGGTTCT	ACAGAGGATTGTGTTGGCTG	20	20	50.0	50.0	58.0	58.1	12.5	0.0	0.106239	112945	114447	1855-1855	0.500000	59.3
ndhD_p4	ndhD	4	GTAGTGATTCACGCGGTTCT	CAGAGGATTGTGTTGGCTGT	20	20	50.0	50.0	58.0	58.1	12.5	0.0	0.106239	112945	114447	1854-1854	0.500000	59.3
ndhD_p5	ndhD	5	GTAGTGATTCACGCGGTTCT	CAGAAACCTCTCCTTGAGCC	20	20	50.0	55.0	58.0	57.9	12.5	0.0	0.114307	112945	114447	1734-1734	0.500000	59.2
ndhE_p1	ndhE	1	ACAGCCAACACAATCCTCTG	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.103779	115031	115336	1123-1134	0.500000	59.5
ndhE_p2	ndhE	2	CAGCCAACACAATCCTCTGT	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.103779	115031	115336	1122-1133	0.500000	59.5
ndhE_p3	ndhE	3	GGCTCAAGGAGAGGTTTCTG	TGGAGTAACTTCCGTGGTCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.111847	115031	115336	1243-1254	0.500000	59.4
ndhE_p4	ndhE	4	AAGGAGAGGTTTCTGCAACG	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.121135	115031	115336	1238-1249	0.500000	59.3
ndhE_p5	ndhE	5	ACATCCGTCCCAAGGTATCA	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.131241	115031	115336	1072-1083	0.500000	59.2
ndhI_p1	ndhI	1	AGACCACGGAAGTTACTCCA	TTTGTGACCTTTGAGGAGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.1	0.024114	116432	116932	1880-1880	0.500000	60.1
ndhI_p2	ndhI	2	AGACCACGGAAGTTACTCCA	GTACGGTTCTGCAATAGGGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.055905	116432	116932	1840-1840	0.500000	59.8
ndhI_p3	ndhI	3	AGACCACGGAAGTTACTCCA	AATAGGGGTGGGAACAGTGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.059117	116432	116932	1828-1828	0.500000	59.8
ndhI_p4	ndhI	4	AGACCACGGAAGTTACTCCA	GACCTTTGAGGAGCCGTATG	20	20	50.0	55.0	58.0	58.1	0.0	0.1	0.064045	116432	116932	1875-1875	0.500000	59.7
ndhI_p5	ndhI	5	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064045	116432	116932	1369-1369	0.500000	59.7
ndhA_p1	ndhA	1	TGACCAATATACCTTGCGGC	AGAGTTGAAGCACCAAAGGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051324	117009	119210	2548-2552	0.500000	59.8
ndhA_p2	ndhA	2	TGACCAATATACCTTGCGGC	AGTTGAAGCACCAAAGGGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051324	117009	119210	2546-2550	0.500000	59.8
ndhA_p3	ndhA	3	TGACCAATATACCTTGCGGC	GAGTTGAAGCACCAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056950	117009	119210	2547-2551	0.500000	59.7
ndhA_p4	ndhA	4	TGACCAATATACCTTGCGGC	GTTGAAGCACCAAAGGGAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056950	117009	119210	2545-2549	0.500000	59.7
ndhA_p5	ndhA	5	TGACCAATATACCTTGCGGC	CAACAGGCGCTAGAAGGAAT	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.068045	117009	119210	2707-2711	0.500000	59.6
rps15_p1	rps15	1	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.085751	120485	120763	794-819	0.500000	63.1
rps15_p2	rps15	2	TGAGGTAAATCAGCAGCGAC	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037273	120485	120763	1225-1250	0.500000	62.2
rps15_p3	rps15	3	TGTTCTGGCGCATTTACAGT	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	10.8	0.0	0.136252	120485	120763	982-1007	0.500000	62.0
rps15_p4	rps15	4	TGAGGTAAATCAGCAGCGAC	GTCCTGTCTTACATCCCCCT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.129204	120485	120763	1164-1164	0.250000	49.0
rps15_p5	rps15	5	AACACCATGCATTGATGGGT	GTCCTGTCTTACATCCCCCT	20	20	45.0	55.0	58.1	58.1	8.9	0.0	0.177682	120485	120763	733-733	0.250000	48.6
ycf1_p1	ycf1	1	CTCAAGCGGCTCCCATTATT	TTGGACGGGGATGTCTTTTG	20	20	50.0	50.0	58.0	58.1	1.4	0.0	0.122841	121454	121573	1044-1044	0.500000	59.2
ycf1_p2	ycf1	2	CTCAAGCGGCTCCCATTATT	TTTGATTGGACGGGGATGTC	20	20	50.0	50.0	58.0	57.9	1.4	0.0	0.149911	121454	121573	1049-1049	0.500000	59.0
ycf1_p3	ycf1	3	TCGCAATTCTCTACGACGTC	TTGGACGGGGATGTCTTTTG	20	20	50.0	50.0	57.8	58.1	2.4	0.0	0.265376	121454	121573	805-805	0.500000	58.2
ycf1_p4	ycf1	4	CGCAATTCTCTACGACGTCT	TTGGACGGGGATGTCTTTTG	20	20	50.0	50.0	57.8	58.1	14.7	0.0	0.271243	121454	121573	804-804	0.500000	58.1
ycf1_p5	ycf1	5	TCGCAATTCTCTACGACGTC	TTTGATTGGACGGGGATGTC	20	20	50.0	50.0	57.8	57.9	2.4	0.0	0.292446	121454	121573	810-810	0.500000	58.0