Hotspot

ndhF-rpl32

SSC · 124319-125310 · 992 bp

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Pi 0.0282
MarkerSeek score 68.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
992
variable_sites
26
pi
0.0282
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGGAAAAAGGAGAAGTCCC TTTGATATAGAAGTGCGTTTTT 55.0 27.3 58.2 52.4 1048-1118 bp 1.0 45.9
ndhF-rpl32_p2 CGGGAAAAAGGAGAAGTCCC TTTTGATATAGAAGTGCGTTTT 55.0 27.3 58.2 52.4 1049-1119 bp 1.0 45.9
ndhF-rpl32_p3 CGGGAAAAAGGAGAAGTCCC TTTTTGATATAGAAGTGCGTTT 55.0 27.3 58.2 52.4 1050-1120 bp 1.0 45.9
ndhF-rpl32_p4 CGGGAAAAAGGAGAAGTCCC TTTTGATATAGAAGTGCGTTTTT 55.0 26.1 58.2 53.3 1049-1119 bp 1.0 45.9
ndhF-rpl32_p5 CGGGAAAAAGGAGAAGTCCC TTTTTGATATAGAAGTGCGTTTT 55.0 26.1 58.2 53.3 1050-1120 bp 1.0 45.9