Hotspot

ycf1-ndhF

SSC · 121998-122071 · 74 bp

Back to Leucaena

Pi 0.108

MarkerSeek score 72.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 74
variable_sites: 8
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GCTCGGGTTATGGAAGAAGG	TAAAATATGCAGGGGGTGGC	55.0	50.0	58.0	57.9	751-795 bp	1.0	80.4
ycf1-ndhF_p2	GCTCGGGTTATGGAAGAAGG	TGTTCATCGGGGTCATAGGA	55.0	50.0	58.0	57.8	1219-1254 bp	1.0	80.2
ycf1-ndhF_p3	AGGGCCCTCTTATCTCTTCC	TAAAATATGCAGGGGGTGGC	55.0	50.0	57.9	57.9	779-823 bp	1.0	79.9
ycf1-ndhF_p4	GGGCCCTCTTATCTCTTCCT	TAAAATATGCAGGGGGTGGC	55.0	50.0	57.9	57.9	778-822 bp	1.0	79.9
ycf1-ndhF_p5	TCGTTGTGGTCGGACTCTAT	TAAAATATGCAGGGGGTGGC	50.0	50.0	58.2	57.9	825-869 bp	1.0	79.3