Hotspot

rpl22-rps19

LSC · 94992-95739 · 748 bp

Back to Leucaena

Pi 0.227

MarkerSeek score 96.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 146
pi: 0.227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 96.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGGCGTATTTGCGTCTTTCA	ATAAACTTCCGGGGACATGC	45.0	50.0	58.5	57.9	792-817 bp	1.0	87.7
rpl22-rps19_p2	ATGGCGTATTTGCGTCTTTC	ATAAACTTCCGGGGACATGC	45.0	50.0	57.2	57.9	793-818 bp	1.0	85.0
rpl22-rps19_p3	GGCGTATTTGCGTCTTTCAT	ATAAACTTCCGGGGACATGC	45.0	50.0	57.2	57.9	791-816 bp	1.0	85.0
rpl22-rps19_p4	TGGCGTATTTGCGTCTTTCA	CTTCCGGGGACATGCAAAAA	45.0	50.0	58.5	59.0	787-812 bp	1.0	79.8
rpl22-rps19_p5	TGGCGTATTTGCGTCTTTCA	TTCCGGGGACATGCAAAAA	45.0	47.4	58.5	57.9	786-811 bp	1.0	79.0