Hotspot

rps8-rpl14

LSC · 91340-92041 · 702 bp

Back to Leucaena

Pi 0.078

MarkerSeek score 81.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 45
pi: 0.078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GCAATGGTGTCCTTACCCAT	GCTCGGGAATTGAGACAGTT	50.0	50.0	57.9	57.9	688-785 bp	1.0	86.9
rps8-rpl14_p2	GATGGTATTCGAACCGTCCC	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.1	57.9	751-848 bp	1.0	86.6
rps8-rpl14_p3	GGTATTCGAACCGTCCCTTT	GCTCGGGAATTGAGACAGTT	50.0	50.0	57.6	57.9	748-845 bp	1.0	84.6
rps8-rpl14_p4	TCAGCAATGGTGTCCTTACC	GCTCGGGAATTGAGACAGTT	50.0	50.0	57.5	57.9	691-788 bp	1.0	84.1
rps8-rpl14_p5	ATGGTATTCGAACCGTCCCT	GCTCGGGAATTGAGACAGTT	50.0	50.0	58.5	57.9	750-847 bp	1.0	83.6