Hotspot

rpl33-rps18

LSC · 78019-78551 · 533 bp

Back to Leucaena

Pi 0.0549

MarkerSeek score 84.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 22
pi: 0.0549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	543-669 bp	1.0	83.8
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	544-670 bp	1.0	83.8
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	545-671 bp	1.0	82.0
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	517-643 bp	1.0	81.2
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	518-644 bp	1.0	81.2