Hotspot

accD-psaI

LSC · 68356-69137 · 782 bp

Back to Leucaena

Pi 0.0488

MarkerSeek score 88.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 33
pi: 0.0488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	897-944 bp	1.0	86.6
accD-psaI_p2	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	903-950 bp	1.0	86.0
accD-psaI_p3	TCAATCGTACCGCGTAATCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	892-939 bp	1.0	85.7
accD-psaI_p4	CAATCGTACCGCGTAATCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	891-938 bp	1.0	85.6
accD-psaI_p5	TCAATCGTACCGCGTAATCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	898-945 bp	1.0	85.0