Hotspot

trnT-UGU-trnL-UAA

LSC · 53639-55532 · 1894 bp

Back to Leucaena

Pi 0.0438

MarkerSeek score 76.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1894
variable_sites: 68
pi: 0.0438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1710-1976 bp	1.0	85.0
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1718-1984 bp	1.0	84.5
trnT-UGU-trnL-UAA_p3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.4	58.0	1682-1948 bp	1.0	84.1
trnT-UGU-trnL-UAA_p4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.4	58.1	1690-1956 bp	1.0	83.6
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1724-1990 bp	1.0	83.1