Hotspot

psbZ-trnG-GCC

LSC · 42986-43732 · 747 bp

Back to Leucaena

Pi 0.049
MarkerSeek score 85.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
747
variable_sites
34
pi
0.049
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.0 57.7 872-902 bp 1.0 84.4
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 875-905 bp 1.0 82.8
psbZ-trnG-GCC_p3 TCCTGATGGTTGGTCGAGTA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 868-898 bp 1.0 82.7
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.3 57.7 873-903 bp 1.0 82.4
psbZ-trnG-GCC_p5 TTCTCCTGATGGTTGGTCGA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 58.4 57.7 871-901 bp 1.0 81.6