Hotspot

atpF-atpH

LSC · 16365-17115 · 751 bp

Back to Leucaena

Pi 0.0328

MarkerSeek score 81.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 23
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	832-869 bp	1.0	81.9
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	899-936 bp	1.0	81.7
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.9	58.6	897-934 bp	1.0	80.2
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	50.0	55.0	57.9	58.6	898-935 bp	1.0	80.2
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	902-939 bp	1.0	79.4