Hotspot

rps16-trnQ-UUG

LSC · 7025-7963 · 939 bp

Back to Leucaena

Pi 0.0426
MarkerSeek score 72.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
939
variable_sites
34
pi
0.0426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 889-1019 bp 1.0 86.2
rps16-trnQ-UUG_p2 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 891-1021 bp 1.0 85.2
rps16-trnQ-UUG_p3 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 877-1007 bp 1.0 84.7
rps16-trnQ-UUG_p4 CATGTCTTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 892-1022 bp 1.0 84.5
rps16-trnQ-UUG_p5 TGTCTTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 890-1020 bp 1.0 83.4