Hotspot

rnl

SSC · 305-3229 · 2925 bp

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Pi 0.00547

MarkerSeek score 34.10

Primer available yes

Label rnl

Feature Metadata

label_name: rnl
feature_type: rRNA
region: SSC
length_bp: 2925
variable_sites: 16
pi: 0.00547
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rnl_p1	TAAACACCAAGACAATATTTTT	GGAGTTAATTTTTAAAGCTAGAA	22.7	26.1	50.2	50.9	3000 bp	0.25	10.0
rnl_p2	TAAACACCAAGACAATATTTTT	GGAGTTAATTTTTAAAGCTAGAAA	22.7	25.0	50.2	51.7	3000 bp	0.25	10.0
rnl_p3	TAAACACCAAGACAATATTTTT	GGAGTTAATTTTTAAAGCTAGAAAT	22.7	24.0	50.2	52.4	3000 bp	0.25	10.0
rnl_p4	TAAACACCAAGACAATATTTTT	GGAGTTAATTTTTAAAGCTAGAAATA	22.7	23.1	50.2	52.4	3000 bp	0.25	10.0
rnl_p5	TAAACACCAAGACAATATTTTT	GGAGTTAATTTTTAAAGCTAGAAATAA	22.7	22.2	50.2	53.2	3000 bp	0.25	10.0