Hotspot

trnT-trnV

SSC · 116590-116882 · 293 bp

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Pi 0.0216

MarkerSeek score 58.00

Primer available yes

Label trnT-trnV

Feature Metadata

label_name: trnT-trnV
feature_type: igs
region: SSC
length_bp: 293
variable_sites: 12
pi: 0.0216
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnV_p1	TTCAAGTCCTTTCACCAGCT	CTGGACTTGAACCAGTGACC	45.0	55.0	57.0	58.1	376-384 bp	1.0	73.4
trnT-trnV_p2	GTTCAAGTCCTTTCACCAGC	CTGGACTTGAACCAGTGACC	50.0	55.0	56.9	58.1	377-385 bp	1.0	73.1
trnT-trnV_p3	TTCAAGTCCTTTCACCAGCT	GGCAGTACTGGACTTGAACC	45.0	55.0	57.0	58.2	383-391 bp	1.0	72.7
trnT-trnV_p4	TTCAAGTCCTTTCACCAGCT	GGGCAGTACTGGACTTGAAC	45.0	55.0	57.0	58.2	384-392 bp	1.0	72.7
trnT-trnV_p5	GTTCAAGTCCTTTCACCAGC	GGCAGTACTGGACTTGAACC	50.0	55.0	56.9	58.2	384-392 bp	1.0	72.4