Hotspot

trnH-trnT

SSC · 116436-116516 · 81 bp

Back to Lessonia

Pi 0.037

MarkerSeek score 70.80

Primer available yes

Label trnH-trnT

Feature Metadata

label_name: trnH-trnT
feature_type: igs
region: SSC
length_bp: 81
variable_sites: 6
pi: 0.037
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-trnT_p1	GTTAAGGCAGTGGGTTGTGA	AGCTGGTGAAAGGACTTGAA	50.0	45.0	58.0	57.0	210 bp	1.0	73.9
trnH-trnT_p2	GTTAAGGCAGTGGGTTGTGA	GCTGGTGAAAGGACTTGAAC	50.0	50.0	58.0	56.9	209 bp	1.0	73.6
trnH-trnT_p3	TATCAAGGTGGGTGTAGCCA	AGCTGGTGAAAGGACTTGAA	50.0	45.0	58.0	57.0	234 bp	1.0	73.5
trnH-trnT_p4	TATCAAGGTGGGTGTAGCCA	GCTGGTGAAAGGACTTGAAC	50.0	50.0	58.0	56.9	233 bp	1.0	73.2
trnH-trnT_p5	TTCAAGTCCCGTCATTCACC	AGCTGGTGAAAGGACTTGAA	50.0	45.0	57.8	57.0	174 bp	1.0	73.1