Hotspot

petF-rpl12

SSC · 107919-108071 · 153 bp

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Pi 0.0545

MarkerSeek score 73.20

Primer available yes

Label petF-rpl12

Feature Metadata

label_name: petF-rpl12
feature_type: igs
region: SSC
length_bp: 153
variable_sites: 16
pi: 0.0545
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF-rpl12_p1	TGACGCCACATTGGGTTTAA	GGTGAAGCAGGGGAAGAAAA	45.0	50.0	58.0	58.0	1030-1054 bp	1.0	82.0
petF-rpl12_p2	TTGACGCCACATTGGGTTTA	GGTGAAGCAGGGGAAGAAAA	45.0	50.0	58.0	58.0	1031-1055 bp	1.0	82.0
petF-rpl12_p3	TGACGCCACATTGGGTTTAA	TCCTGGAGTAAAGGGACGTT	45.0	50.0	58.0	58.0	1338-1362 bp	1.0	81.7
petF-rpl12_p4	GGTGCTTGTTCATCATGTGC	GGTGAAGCAGGGGAAGAAAA	50.0	50.0	58.0	58.0	555 bp	0.25	49.9
petF-rpl12_p5	GGTGCTTGTTCATCATGTGC	TCCTGGAGTAAAGGGACGTT	50.0	50.0	58.0	58.0	863 bp	0.25	49.7