Hotspot

psbN-psbT

SSC · 105704-105757 · 54 bp

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Pi 0.0463

MarkerSeek score 70.40

Primer available yes

Label psbN-psbT

Feature Metadata

label_name: psbN-psbT
feature_type: igs
region: SSC
length_bp: 54
variable_sites: 5
pi: 0.0463
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbT_p1	GGCCGGGGTCAAAATTTTTG	AGTAGTCCACGTGGTTGGTA	50.0	50.0	58.5	58.0	460-496 bp	1.0	77.6
psbN-psbT_p2	GCCGGGGTCAAAATTTTTGA	AGTAGTCCACGTGGTTGGTA	45.0	50.0	57.5	58.0	459-495 bp	1.0	77.2
psbN-psbT_p3	GGCCGGGGTCAAAATTTTTG	GAATCGCAGAGGCTTGGTTA	50.0	50.0	58.5	58.0	789-825 bp	1.0	76.9
psbN-psbT_p4	GGCCGGGGTCAAAATTTTTG	AATCGCAGAGGCTTGGTTAG	50.0	50.0	58.5	58.0	788-824 bp	1.0	76.9
psbN-psbT_p5	GGCCGGGGTCAAAATTTTTG	TCAGAAGTAGTCCACGTGGT	50.0	50.0	58.5	58.0	465-501 bp	0.5	56.9