Hotspot

rbcR-psbY

LSC · 80699-81113 · 415 bp

Back to Lessonia

Pi 0.0217

MarkerSeek score 57.40

Primer available yes

Label rbcR-psbY

Feature Metadata

label_name: rbcR-psbY
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 17
pi: 0.0217
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcR-psbY_p1	TGTTCAAGGTTAAATGGGAA	AGGAGCAAAAACAAATATAAGACGT	35.0	32.0	52.5	57.6	477-485 bp	1.0	42.8
rbcR-psbY_p2	TTGTTCAAGGTTAAATGGGA	AGGAGCAAAAACAAATATAAGACGT	35.0	32.0	52.5	57.6	478-486 bp	1.0	42.8
rbcR-psbY_p3	TGTTCAAGGTTAAATGGGAAA	AGGAGCAAAAACAAATATAAGACGT	33.3	32.0	53.5	57.6	477-485 bp	1.0	42.8
rbcR-psbY_p4	TTGTTCAAGGTTAAATGGGAA	AGGAGCAAAAACAAATATAAGACGT	33.3	32.0	53.5	57.6	478-486 bp	1.0	42.8
rbcR-psbY_p5	TTGTTCAAGGTTAAATGGGAAA	AGGAGCAAAAACAAATATAAGACGT	31.8	32.0	54.3	57.6	478-486 bp	1.0	42.8