Hotspot

rps18-rpl33

LSC · 69364-69373 · 10 bp

Back to Lessonia

Pi 0.05
MarkerSeek score 62.60
Primer available yes
Label rps18-rpl33

Feature Metadata

label_name
rps18-rpl33
feature_type
igs
region
LSC
length_bp
10
variable_sites
1
pi
0.05
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl33_p1 TGACGTCTAGTTTTTGTTGGC TGTCCGAACTGTAATTCACA 42.9 40.0 57.0 54.6 102 bp 1.0 40.8
rps18-rpl33_p2 TGACGTCTAGTTTTTGTTGGC TTGTCCGAACTGTAATTCACA 42.9 38.1 57.0 55.5 103 bp 1.0 40.8
rps18-rpl33_p3 GACGTCTAGTTTTTGTTGGCT TGTCCGAACTGTAATTCACA 42.9 40.0 56.8 54.6 101 bp 1.0 40.8
rps18-rpl33_p4 TTGACGTCTAGTTTTTGTTGGC TGTCCGAACTGTAATTCACA 40.9 40.0 57.7 54.6 103 bp 1.0 40.8
rps18-rpl33_p5 TGACGTCTAGTTTTTGTTGGC TGTCCGAACTGTAATTCACAT 42.9 38.1 57.0 55.3 102 bp 1.0 40.8