Hotspot

psaJ-cp74

LSC · 62736-62893 · 158 bp

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Pi 0.0462

MarkerSeek score 72.10

Primer available yes

Label psaJ-cp74

Feature Metadata

label_name: psaJ-cp74
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 12
pi: 0.0462
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-cp74_p1	TGGCAAGAATTCACTACGGG	CGCCGATATAGCTTTCCCAT	50.0	50.0	57.9	57.6	797-816 bp	1.0	81.2
psaJ-cp74_p2	GGCAAGAATTCACTACGGGT	CGCCGATATAGCTTTCCCAT	50.0	50.0	57.9	57.6	796-815 bp	1.0	81.2
psaJ-cp74_p3	TTTGGCCAGTATCAGCTTGG	CGCCGATATAGCTTTCCCAT	50.0	50.0	58.2	57.6	814-833 bp	1.0	80.8
psaJ-cp74_p4	TGGGCTGGTCGTACTTATCT	CGCCGATATAGCTTTCCCAT	50.0	50.0	57.8	57.6	923-942 bp	1.0	80.4
psaJ-cp74_p5	TGGCAAGAATTCACTACGGG	CAACCTCAAGTGCATGCTCT	50.0	50.0	57.9	58.5	925 bp	0.25	45.4