Hotspot

trnS-psaD

LSC · 24723-24921 · 199 bp

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Pi 0.0392

MarkerSeek score 68.80

Primer available yes

Label trnS-psaD

Feature Metadata

label_name: trnS-psaD
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 13
pi: 0.0392
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psaD_p1	ACGCGTAAACGTTCATCTGA	ACCCAGCTTCTATAAAATGGA	45.0	38.1	57.9	54.3	273-285 bp	1.0	49.6
trnS-psaD_p2	AAATCAGACGCAATCGACCA	ACCCAGCTTCTATAAAATGGA	45.0	38.1	57.9	54.3	249-261 bp	1.0	49.6
trnS-psaD_p3	ATCAGACGCAATCGACCATC	ACCCAGCTTCTATAAAATGGA	50.0	38.1	58.1	54.3	247-259 bp	1.0	49.2
trnS-psaD_p4	ACGCGTAAACGTTCATCTGA	CCTAACCCAGCTTCTATAAAATGG	45.0	41.7	57.9	57.1	277-289 bp	1.0	48.2
trnS-psaD_p5	ACGCGTAAACGTTCATCTGA	ACCCAGCTTCTATAAAATGGAA	45.0	36.4	57.9	55.1	273-285 bp	1.0	48.2