Hotspot

rpl32-trnL-UAG

SSC · 109231-109777 · 547 bp

Back to Lespedeza

Pi 0.00904
MarkerSeek score 51.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
547
variable_sites
20
pi
0.00904
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACGGGGAATTCAAAAAGCTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 56.1 57.9 631-642 bp 1.0 67.3
rpl32-trnL-UAG_p2 ACGGGGAATTCAAAAAGCTT AGCAGCGTGTCTACCAATTT 40.0 45.0 56.1 57.8 624-635 bp 1.0 66.8
rpl32-trnL-UAG_p3 ACGGGGAATTCAAAAAGCTT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 56.1 57.8 630-641 bp 1.0 66.7
rpl32-trnL-UAG_p4 ACGGGGAATTCAAAAAGCTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 56.1 57.8 629-640 bp 1.0 66.7
rpl32-trnL-UAG_p5 ACGGGGAATTCAAAAAGCTT GAGCAGCGTGTCTACCAATT 40.0 50.0 56.1 58.3 625-636 bp 1.0 66.1