Hotspot

accD-psaI

LSC · 57552-57905 · 354 bp

Back to Lespedeza

Pi 0.0116

MarkerSeek score 46.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 14
pi: 0.0116
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	467-519 bp	1.0	86.1
accD-psaI_p2	TTGATTCAATCGTACCGCGT	ATAGAAACACATCGTGGCCC	45.0	50.0	58.0	58.0	926-989 bp	1.0	85.7
accD-psaI_p3	TTGATTCAATCGTACCGCGT	CCCCTTGCGGAATAAATGGA	45.0	50.0	58.0	57.9	909-972 bp	1.0	85.6
accD-psaI_p4	AGGTGGGGTAACAGCTAGTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	628-680 bp	1.0	85.4
accD-psaI_p5	TTGATTCAATCGTACCGCGT	ACAGCGACCGATAACAATCC	45.0	50.0	58.0	58.1	850-871 bp	0.888889	77.3