Hotspot

trnQ-UUG-rps16

LSC · 54036-54453 · 418 bp

Back to Lespedeza

Pi 0.023
MarkerSeek score 66.40
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
418
variable_sites
28
pi
0.023
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAGGTTCGAATCCTTCCGTC GTCTTTCAAGTCGCACGTTG 55.0 50.0 58.1 58.0 495-505 bp 1.0 85.9
trnQ-UUG-rps16_p2 GTCAAATCGAAAATGGGGCG GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.1 58.0 556-566 bp 1.0 85.1
trnQ-UUG-rps16_p3 GAGGTTCGAATCCTTCCGTC ATGTCTTTCAAGTCGCACGT 55.0 45.0 58.1 58.1 497-507 bp 1.0 84.9
trnQ-UUG-rps16_p4 GAGGTTCGAATCCTTCCGTC GCACGTTGCTTTTTACCACA 55.0 45.0 58.1 57.8 483-493 bp 1.0 84.6
trnQ-UUG-rps16_p5 GTCAAATCGAAAATGGGGCG ATGTCTTTCAAGTCGCACGT 50.0 45.0 58.1 58.1 558-568 bp 1.0 84.0