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psbI-psbK

LSC · 52894-53349 · 456 bp

Back to Lespedeza

Pi 0.0107

MarkerSeek score 41.60

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 19
pi: 0.0107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	TCACTACCGTGTAAACAAATAGT	CTTTTGTTTGGCAAGCTGCT	34.8	45.0	55.6	58.1	528-555 bp	1.0	42.9
psbI-psbK_p2	TCACTACCGTGTAAACAAATAGT	GTTTGGCAAGCTGCTGTAAG	34.8	50.0	55.6	57.9	523-550 bp	1.0	42.9
psbI-psbK_p3	ATCACTACCGTGTAAACAAA	CTTTTGTTTGGCAAGCTGCT	35.0	45.0	52.5	58.1	529-556 bp	1.0	42.9
psbI-psbK_p4	TCACTACCGTGTAAACAAAT	CTTTTGTTTGGCAAGCTGCT	35.0	45.0	52.5	58.1	528-555 bp	1.0	42.9
psbI-psbK_p5	TCACTACCGTGTAAACAAAT	GTTTGGCAAGCTGCTGTAAG	35.0	50.0	52.5	57.9	523-550 bp	1.0	42.9