Hotspot

trnG-UCC-trnS-GCU

LSC · 52096-52520 · 425 bp

Back to Lespedeza

Pi 0.00843

MarkerSeek score 56.70

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 16
pi: 0.00843
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	GGGGTCCATAGAACGAATCG	CAATCCGACGCTTTAGTCCA	55.0	50.0	57.8	58.0	504-528 bp	1.0	85.2
trnG-UCC-trnS-GCU_p2	GGGGTCCATAGAACGAATCG	AATCCGACGCTTTAGTCCAC	55.0	50.0	57.8	58.0	503-527 bp	1.0	85.2
trnG-UCC-trnS-GCU_p3	GGTCCATAGAACGAATCGCA	CAATCCGACGCTTTAGTCCA	50.0	50.0	57.8	58.0	502-526 bp	1.0	84.7
trnG-UCC-trnS-GCU_p4	GGTCCATAGAACGAATCGCA	AATCCGACGCTTTAGTCCAC	50.0	50.0	57.8	58.0	501-525 bp	1.0	84.7
trnG-UCC-trnS-GCU_p5	GGGGTCCATAGAACGAATCG	CAACGATCAGCAACGGAAAC	55.0	50.0	57.8	58.0	575-599 bp	1.0	84.6