Hotspot

atpH-atpF

LSC · 47295-47874 · 580 bp

Back to Lespedeza

Pi 0.00398

MarkerSeek score 55.80

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 8
pi: 0.00398
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TGGGCTGGTTGTAGCATTAG	GCCAATAGCCCAAACAAAGG	50.0	50.0	57.6	57.6	660-672 bp	1.0	77.3
atpH-atpF_p2	CCAGAGGCAGAGGGAAAAAT	GCCAATAGCCCAAACAAAGG	50.0	50.0	57.5	57.6	731-743 bp	1.0	76.1
atpH-atpF_p3	TGGGCTGGTTGTAGCATTAG	TTAAACCCGAAACTTCCGGC	50.0	50.0	57.6	58.8	685-697 bp	1.0	74.2
atpH-atpF_p4	TTTATGGGCTGGTTGTAGCA	GCCAATAGCCCAAACAAAGG	45.0	50.0	57.1	57.6	664-676 bp	1.0	73.5
atpH-atpF_p5	CCAGAGGCAGAGGGAAAAAT	TTAAACCCGAAACTTCCGGC	50.0	50.0	57.5	58.8	756-768 bp	1.0	73.0