Hotspot

trnE-UUC-psbM

LSC · 30774-31218 · 445 bp

Back to Lespedeza

Pi 0.00738

MarkerSeek score 57.00

Primer available yes

Label trnE-UUC-psbM

Feature Metadata

label_name: trnE-UUC-psbM
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 12
pi: 0.00738
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbM_p1	TCAACTTCCGTTCGTAGCAC	AGGAACTAGAATAAACAGTGCAGT	50.0	37.5	58.2	57.9	591-607 bp	1.0	48.1
trnE-UUC-psbM_p2	GCTGCCTCCTTGAAAGAGAG	AGGAACTAGAATAAACAGTGCAGT	55.0	37.5	58.3	57.9	544-560 bp	1.0	48.0
trnE-UUC-psbM_p3	TGTCCTGAACCACTAGACGA	AGGAACTAGAATAAACAGTGCAGT	50.0	37.5	57.7	57.9	523-539 bp	1.0	48.0
trnE-UUC-psbM_p4	CTGAACCACTAGACGATGGG	AGGAACTAGAATAAACAGTGCAGT	55.0	37.5	57.4	57.9	519-535 bp	1.0	45.6
trnE-UUC-psbM_p5	TCAACTTCCGTTCGTAGCAC	GGAACTAGAATAAACAGTGCAGT	50.0	39.1	58.2	56.5	590-606 bp	1.0	45.4