Hotspot

trnS-UGA-psbC

LSC · 25665-25891 · 227 bp

Back to Lespedeza

Pi 0.0323

MarkerSeek score 56.70

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 21
pi: 0.0323
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TGATCGAGATTTGGAACCTGT	55.0	42.9	58.0	57.0	365 bp	1.0	68.6
trnS-UGA-psbC_p2	GAGAGATGGCTGAGTGGTTG	TCGAGATTTGGAACCTGTTCT	55.0	42.9	58.0	57.0	362 bp	1.0	68.1
trnS-UGA-psbC_p3	ATAGCCCCGGTCTTGAAAAC	TGATCGAGATTTGGAACCTGT	50.0	42.9	57.9	57.0	345 bp	1.0	68.0
trnS-UGA-psbC_p4	GCTTTTGGAGAGATGGCTGA	TGATCGAGATTTGGAACCTGT	50.0	42.9	57.9	57.0	372 bp	1.0	67.7
trnS-UGA-psbC_p5	AGCTTTTGGAGAGATGGCTG	TGATCGAGATTTGGAACCTGT	50.0	42.9	57.9	57.0	373 bp	1.0	67.6